Printing will take place in various locations across Canada – Halifax, Montreal, Toronto and Vancouver. The contract will come into effect in 2010 and will last until 2028.
Transcontinental said it would invest about $200m during 2009-2010 in a new Canada-wide platform for newspaper and flyer printing.
Phillip Crawley, publisher and chief executive officer of The Globe and Mail, said: "High-quality colour production capacity has always been a key point of distinction for The Globe and Mail.
"Transcontinental's commitment to providing the most progressive high-speed colour printing capability will keep us at the leading edge."
Transcontinental also produces The New York Times for the upper New York state and Ontario markets.
Transcontinental seals major deal with Canadian newspaper
North American print giant Transcontinental has landed a major contract with a Canadian national newspaper <i>The Globe and Mail</i> worth around $1.7bn (900m).