At present the Marie Curie hospice at Huntershill has raised £12.6m of its £16.1m target, with £25,000 of print donated by the Scottish printer.
The hospice was built 30 years ago but its facilities are dated. The money raised will be put towards building a replacement hospice in the grounds of a nearby hospital.
John Watson said that the gift was in line with the company's "long standing commitment to social justice".
He added: "We have been very fortunate in business and value greatly the success which hard work has brought.
"But we readily acknowledge that with great success comes a responsibility to the community and I regard our charity work as a vital component of the way we do business.
Watson was awarded an OBE for services to printing and charity in 2006.
To donate to the hospice, click here for more information.
Scottish printer donates 25,000 print to local hospice
John Watson and Co has provided free print for a fundraising campaign to build a new hospice in Glasgow.
