The Manchester-based superwide-format specialist printed the graphic directly on Clearvision material with an HP Scitex TJ8300 press.
The 40mx15m display, which was commissioned by the club's sponsor AIG, took a day to print and cut in-house before it was installed over the course of a week at the club's ground, Old Trafford.
Three MediaCo staff were involved in installing the Clearvision substrate, a perforated film material that allows light to flood through, onto the glass of the building's façade.
The image depicts the Busby Babes' final line-up with the words of terrace favourite 'United Calypso' reflected opposite.
MediaCo managing director Mark Wardle said: "This has been a particularly poignant project to work on. MediaCo has always been a Manchester-based company and the Busby Babes are true heroes in this city."
He added: "We are very proud to be involved in producing this spectacular tribute."
MediaCo pays tribute to Man U's Busby Babes with Old Trafford display
MediaCo has produced a full-colour window graphic for Manchester United to mark the 50th anniversary of the plane crash that killed 23 people associated with the club, including eight of the 'Busby Babes'.