The title, launched in 2007, had a circulation of 11,500 and brought the US concept of local business weeklies to the UK.
However, the publisher cited poor support from advertisers as a key factor behind the decision to close, which editor-in-chief Chris Crain said "made the project unsustainable.
The company could not be reached for comment at the time of writing.
Crain's Manchester Business closes
Crain Communications has closed Crain's Manchester Business after three years.